recombinant – Communicable Diseases Research

A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine

Article

Published on 2022-12-012022-11-15
Journal: Emerging Microbes & Infections
[Category] COVID19(2023년), SARS, 변종, 진단,

[키워드] acute respiratory syndrome analysed antibody antibody titres BALB/c BALB/c mouse boost CD4 CD8 cellular cellular immunity coronavirus coronavirus disease COVID-19 COVID-19 vaccine Delta/B.1.617.2 variant of concern effective eight elicited emerging and re-Emerging coronaviruses glycoprotein homologous humoral Humoral immunity immunization Japanese LC16m8 less mice motif multifunctional neutralized Neutralizing activity patients with COVID-19 polybasic prime-boost immunization. provide recombinant replication-competent viral S-specific antibody S1/S2 cleavage site SARS-CoV-2 serum sample significantly lower Strains T-cell immune responses T-cell immunity T-cell Response the S protein Vaccine Vaccine development vaccine platform variant vector viral vectors virus Wuhan
[DOI] 10.1080/22221751.2022.2122580 PMC 바로가기

Hamsters Protected from SARS-CoV-2 Delta Variant Challenge after Two Doses of Adjuvanted SARS-CoV-2 Recombinant Spike Protein (S-2P) and One Dose of Beta S-2P

Article

Published on 2022-11-012022-11-15
Journal: The Journal of Infectious Diseases
[Category] SARS, 변종, 진단,

Monitoring SARS-CoV-2 Infection Using a Double Reporter-Expressing Virus

Article

Published on 2022-10-262022-11-15
Journal: Microbiology Spectrum
[Category] COVID19(2023년), SARS, 진단, 치료기술, 치료제,

[키워드] acute respiratory syndrome animal models antiviral therapeutics antivirals biology of SARS-CoV-2 Care caused comparable contagious coronavirus coronavirus disease COVID-19 cultured cells demonstrated detect Express expresse expressing expression level facilitate feasibility fluorescent Genes hACE2 hamster Health human Angiotensin-converting enzyme in vitro In vitro assay in vivo infected cell Infection K18 knowledge limitation luciferase luciferase reporter gene luciferase reporter genes mCherry monitoring neutralizing antibody overcome pandemic pathogenicity Protein recombinant reporter responsible robust SARS-CoV-2 SARS-CoV-2 biology SARS-COV-2 infection transgenic mice Transmission Transmission. Vaccine viral fitness viral infection virus wild-type
[DOI] 10.1128/spectrum.02379-22 PMC 바로가기

Recombinant Protein Micelles to Block Transduction by SARS-CoV-2 Pseudovirus

Article

Published on 2022-10-252022-11-16
Journal: ACS Nano
[Category] COVID19(2023년), SARS, 변종,

As the virus evolves, so too must we: a drug developer’s perspective : We need a new paradigm in searching for next-generation countermeasures

Article

Published on 2022-10-102022-11-15
Journal: Virology Journal
[Category] COVID19(2023년), SARS, 변종, 치료제,

A fast, ultrasensitive SERS immunoassay to detect SARS-CoV-2 in saliva

Article

Published on 2022-10-092022-11-15
Journal: Analytica chimica acta
[Category] COVID19(2023년), SARS, 변종, 진단, 치료기술,

A Recombinant VSV-Based Bivalent Vaccine Effectively Protects against Both SARS-CoV-2 and Influenza A Virus Infection

Article

Published on 2022-09-282022-11-15
Journal: Journal of Virology
[Category] SARS, 변종, 신종인플루엔자, 진단,

[키워드] Amino acid animal Bivalent bivalent vaccine C-terminal Cellular immune response conserved contagious COVID-19 Delta delta variant develop domain Ebola ectodomain Efficacy Evidence fused global public health glycoprotein H1N1 H3N2 hamster humoral immunization infections Infectious disease Influenza influenza A virus influenza virus influenza viruses M2 protein mice Neutralizing neutralizing antibody Point mutation PROTECT Protein provide recombinant reduced Respiratory disease respiratory droplet respiratory viral S2 domain SARS-CoV-2 SARS-CoV-2 Delta variant SARS-CoV-2 SP SARS-CoV-2 variant shown significantly Spike protein spike protein. the RBD threat transmitted Vaccine vaccine candidate vaccine platform variant vesicular stomatitis virus VSV VSV vector
[DOI] 10.1128/jvi.01337-22 PMC 바로가기

Molecular Analyses of Clinical Isolates and Recombinant SARS-CoV-2 Carrying B.1 and B.1.617.2 Spike Mutations Suggest a Potential Role of Non-Spike Mutations in Infection Kinetics

Article

Published on 2022-09-122022-11-15
Journal: Viruses
[Category] SARS, 변종, 진단,

[키워드] acute respiratory syndrome addition analysis B.1 B.1.617.2 BAC BAC mutagenesis backbone Bacterial bronchial epithelial cells Carrying chromosome clinical comparable coronavirus Cytopathic effect disease Efficiency en passant mutagenesis epithelial cell line evaluate generate glycoprotein human lung Infection infectious virus inhibitors isolate isolates Kinetics less Microscopy monoclonal antibody Mutagenesis Mutation neutralization nonstructural proteins nucleocapsid Numerous Post-vaccine Potential progression raise recombinant RNA role SARS-CoV-2 sera severity spike spike mutation spike mutations Spike protein spike protein. Substitution suggested susceptible Syncytia Transmissibility Treatment variant viral fitness viral spike virus Virus life cycle VoC
[DOI] 10.3390/v14092017 PMC 바로가기

SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis

Article

Published on 2022-09-092022-11-16
Journal: International Journal of Molecular Sciences
[Category] SARS, 변종,

[키워드] ACE2 acute respiratory distress alveolar angiotensin-converting enzyme 2 binding blocked Blood coagulation Capillaries cell types cellular co-culture Coagulopathy communication Contact COVID-19 COVID-19 patient Delta Disease progression drug DTNB endothelial Endothelial cell endotheliopathy Evidence Express expression extrinsic pathway Factor Factor-V fibrinogen functional groups had no Human human lung human lung endothelial cells humans induce infect inhibited inhibitor interactions investigated leukocyte mechanism Microvascular thrombosis neutrophil Neutrophils Pathogenesis pathway Patient potential mechanisms prevented Protein pulmonary complication Pulmonary endothelium recombinant S-protein SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike proteins SARS-CoV-2 variant SARS-CoV-2 virion secretion severe lung injury significantly increased spike syndrome target cell TFPI therapeutic strategy thrombin thrombomodulin thrombosis tissue Tissue factor. Transcriptional upregulation vascular endothelial cell vessel Wuhan
[DOI] 10.3390/ijms231810436 PMC 바로가기

Recombinant COVID-19 vaccine based on recombinant RBD/Nucleoprotein and saponin adjuvant induces long-lasting neutralizing antibodies and cellular immunity

Article

Published on 2022-09-082022-11-15
Journal: Frontiers in Immunology
[Category] SARS, 진단, 치료기술,

[키워드] caused CD4+ CD4+ and CD8+ CD8+ T cell Cell cellular immunity Combination COVID-19 vaccine cytokine domain doses E. coli effective elicit elicited ELISA expressed female flow cytometry global pandemic granzyme groups IFN-γ IgG IgG1 IgG2a IL-12 IL-4 immune response immunized increase in induce infecting isotype long-lasting long-lasting neutralizing antibody lymphoproliferative mice N protein neutralization neutralization antibodies Neutralization antibody nucleocapsid percentage promoted Protective purified purified RBD quantified RBD recombinant Recombinant protein responses robust Saponin SARS-CoV-2 SARS-CoV-2 vaccine significantly spleen subcutaneously surrogate sVNT T cell response Test the SARS-CoV-2 Vaccine vaccine candidates vaccine group vaccine. virus were measured
[DOI] 10.3389/fimmu.2022.974364 PMC 바로가기